1. Use existing trucks as a reference
While real-life trucks look more or less identical to the casual observer, there are quite a few differences between your average short-range truck and a long-hauler used for transporting goods over long distances.
Short-range trucks rarely contain any amenities, while long-haulers may contain a bed, power outlets (for shavers, laptops etc.), microwave oven and sometimes a dry toilet and even a small sink and table.
Try looking up trucks with "sleeper cabs" for some ideas on their configuration - They range from rather basic (just a bed) to full-on luxury caravan configurations.
2. The economics of size, speed and regulations
Much of this depends on what constitutes a "short" or "long" haul in your universe. Since you do mention FTL, I also assume that your company will handle at least some interstellar haulage.
Let's take a real-life example first - let's say a fairly long trip like Copenhagen, Denmark to Milan, Italy:
The trip by normal car takes 17-18 hours, however regulations state that not only are trucks limited in speed (max 80km/h), but also that the trucker has to take regular rests (regular breaks at least every 4½ hours lasting 45 minutes and an at least 11-hour "daily rest" period)
Considering a round trip of this magnitude will take ~4 whole days in and of itself (and that only if road conditions are perfect), at least some basic amenities are needed either in the truck or rest stops along the way - some things are considered basic or essential, such as a bed in the truck.
Also keep in mind that longer hauls also often are driven with double trailers This allows the trucker to pass multiple waypoints or transport larger amounts of goods at once
Moving this to your "space trucker" scenario, you need to consider how long it takes to get to specific places. Truckers do want to get home after a while, so also consider round trips. How long does it take to the Alpha Centauri system? Barnard's Star? Vega?
The longer a haul is, the more you have to think about amenities for your space truckers. If the trip to at least the nearest rest station lasts for more than a few hours, that crew will at the very least need a toilet!
Depending on how you plan your FTL works, it may provide benefits or caveats in how much haulage a space truck can carry. A jump-gate system may offer a better option for large hauls, while Alcubierre/warp travel may be affected negatively by a large amount of mass on board.
3. The economy of rest stops
On Earth, rest stops are usually placed within maybe an hour's truck drive from each other, but they are also within range of electricity, water supply etc.
It would be vastly impractical (and expensive) to erect space stations in the void between stars for a multitude of reasons. At least inside a star system, you will have at least a few planets in range for mining ice (for water) and materials, as well as a star to harvest energy from.
Then again, there's no reason these stations would function solely as rest stops and laybys for space truckers - that again would be impractical due to the potential resources in a given star system.
4. Life boats
This depends entirely on how well-formed the infrastructure is. If the spacelanes in the area are well-trafficked, then a simple pod with a distress beacon and enough food, water and oxygen for 2 for ~48 or 72 hours should suffice.
Though if the trucker operates in less well-developed areas of space, there should be some more precautions taken for the pod - eg. the ability to seek out nearest station or planet, at least to a degree.
All in all life boats (and their condition) would depend on regulations and/or how "shady" your interstellar shipping company is.
The layout
Real life trucks come in a multitude of configurations for different situations, and most truck dealerships allow you to build your own truck to a certain degree with a given engine, cab, tank size etc.. They all usually base on the same platform, though.
This would likely be the same for "space trucks". You have a base model where buyers can configure them for different workloads - a "long hauler" will be able to carry more fuel and have a more powerful STL/FTL engine setup, as well as a larger and more "comfortable" or practical cabin for the crew.
For cargo, I'd imagine the "space trucks" can use something akin to a standard shipping container with protection (eg. air or heat) etc. implemented as needed for the cargo inside. These should be accessible directly or even detachable.
The cargo medium (containers) is adaptable to a large set of vehicles - usable on the space truck, transferable via shuttle or space drones and usable on the ground aboard ships and "normal" trucks. All without using precious time to unload and reload cargo.
Much like a normal truck, the cockpit and engine assembly would preferably be concentrated to one spot so the crew can perform some degree of repairs from the inside of the ship.
The cabin does not need to be clad in wood and leather, but it would need at least:
- a toilet (bath is optional)
- a pair of (bunk) beds
- small microwave cubby (see images of truck sleeper cabs how that would work)
- If cabin is zero-G, some exercise equipment to prevent your truckers from atrophy.
- probably some seating and a table
- storage options for food, spare parts and their own amenities.
The Crew
It's a pretty sound idea that "space truckers" would operate in pairs - though, I'd suggest that both are trained in some degree in engineering. That way, they can both operate in shifts, or in tandem if the situation demands it.
The crew will likely have to be able to work with each other for days or even a few weeks, so there may likely be a shift rotation regulation in place, so that one of them can remain awake should there be any problems on the route.