If what you want is an actual separate species or subspecies you have to have reproductive isolation which you will never get if they are kidnapping men to breed with, simple as that.
There's only one way to achieve an actual subspecies (something beginning on the path to actual speciation but not quite there yet) that's all female, parthenogenesis.
Anything where they breed with normal humans to get pregnant but only birth females (the effect of some form of dominant gene resulting in only female conceptions or early miscarriage or still birth of any males) isn't technically speaking a subspecies, it's the same species, same goes for if they simply get rid of male births by infanticide which would be cultural not genetics anyway.
The constant influx of DNA from other human populations in your example would ensure any form of speciation would never happen, simple as that.
So you're probably looking at some sort of random mutation as in whiptail lizards to start off with followed by cultural isolation ('men bad, we don't want men') to allow speciation (or sub speciation if you prefer) to begin to develop.
If you still really want them to kidnap men then try this on for size, Whiptail lizards often go through the motions of mating with each other even though no genetic material is exchanged (both being female), it's just an instinctive pantomime that helps get the hormones that trigger pregnancy going for them (those that do this tend to be more fertile), so perhaps have your Amazons fully speciated such that they can no longer breed with other humans, the sperm won't penetrate the egg, but the act of sex triggers hormonal changes making their parthenogenic pregnancy more likely & sex with men works better than doing it for yourself or with other women, they may not even realise the men are making zero contribution to the offspring, they could be fully speciated in this way & still look identical to normal humans.
So the question is do you really want a subspecies or just a cultural thing with a bit of a twist, which has already been done to death in fiction anyway.
Alternatively just have them lie about being a subspecies & not having any male births & toss any boy babies off the cliff out back of the house when no one's looking, much simpler.